human es cell lines h1 wa01  (WiCell Research Institute Inc)

Journal: International Journal of Molecular Sciences

Article Title: Impact of AHR Ligand TCDD on Human Embryonic Stem Cells and Early Differentiation

doi: 10.3390/ijms21239052

Figure Lengend Snippet: Expression of AHR and pluripotency marker genes in hESCs and embryoid bodies (EBs) cultured for 5 days. ( a ) Representative Western blot and densitometry analysis of AHR and actin protein levels in H9 cells. qPCR analysis of AHR ( b ), OCT4 , SOX2 and NANOG ( c ) mRNA levels in H1 and H9 hESCs and EBs. Data are presented relative to hESC H9 as means ± SEM from three independent experiments. * p < 0.05; ** p < 0.01.

Article Snippet: Human ES cell lines H1 (46, XY, WA01) and H9 (46, XX, WA09) were obtained from WiCell Research Institute (National Stem Cell Bank, Madison, WI, USA).

Techniques: Expressing, Marker, Cell Culture, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Impact of AHR Ligand TCDD on Human Embryonic Stem Cells and Early Differentiation

doi: 10.3390/ijms21239052

Figure Lengend Snippet: Analysis of lineage-specific marker gene expression in DMSO or TCDD pre-treated H9 hESCs and during directed differentiation into neural, endo- and mesodermal lineages. qPCR analysis of neural marker genes PAX6 ( a ) and OTX2 ( b ), endodermal marker genes SOX17 ( c ) and GATA4 ( d ) and mesodermal marker genes T ( e ) , HAND1 ( f ) and GSC ( g ) mRNA levels. Data are presented relative to hESC DMSO ( a , b , e , g ) or d2 DMSO ( c , d , f ) as means ± SEM from three independent experiments. * p < 0.05 vs. hESC; ** p < 0.01 vs. hESC; # p < 0.05 vs. d2; ## p < 0.01 vs. d2.

Article Snippet: Human ES cell lines H1 (46, XY, WA01) and H9 (46, XX, WA09) were obtained from WiCell Research Institute (National Stem Cell Bank, Madison, WI, USA).

Techniques: Marker, Expressing

Journal: Neuron

Article Title: Neuroligin-4 regulates excitatory synaptic transmission in human neurons

doi: 10.1016/j.neuron.2019.05.043

Figure Lengend Snippet: (A) Representative images of patch clamping of EGFP labeled AD-iN cells in mixed cultures. (B) Quantification of intrinsic properties of spiked-in AD-iN cells in mixed cultures differentiated from wild-type H1 (WT), NLGN4KO (KO) and NLGN4R704C (R704C) cells. (C) Example mPSCs traces recorded in the presence of TTX, (D) quantification of mIPSCs amplitudes and frequencies, (E) quantification of mEPSCs amplitudes and frequencies from spiked-in AD-iN cells WT, KO and R704C at day42. (F) Example traces and quantification of sEPSCs and (G) evoked EPSC amplitudes recorded from AM-iN cells WT, KO and R704C cells at day42. (H) Representative images of surface (live staining) and total HA signal (fixed staining) in day 28 Ngn2 iN cells expressing NGLN4-HA or NLGN4R704C-HA. (I) Quantification of surface localized NLGN4 relative to total NLGN4 in Ngn2 iN cells (left) expressing NGLN4-HA (WT) or NLGN4R704C-HA (R704C). Right panel, immunoblotting from the same cultures shows comparable levels of total NLGN4 protein in WT and R704C. (J) NLGN4 co-immunoprecipitates with GluA1 and PSD-95. The R704C mutation enhances co-immunoprecipitation of NLGN4 with GluA1. Protein lysates from Ngn2 iN cells expressing NLGN4 WT, R704C or EGFP (Ctrl) immunoprecipitated with HA antibodies and blotted for the AMPAR-GluA1 and for PSD-95. Left, quantification of the relative levels of GluA1 and PSD95 in the immunoprecipitates. Scale bars: 50μm for panel A and upper panel H and 10μm lower panel H. Data are represented as mean ± SEM and N=3. Numbers of neurons/ independent cultures analyzed are shown in the bars. (*, p < 0.05) (***, p < 0.001). See also Figure S4.

Article Snippet: Male human embryonic stem (ES) cells line H1 (WA01 WiCell Research Institute, Inc.) were cultured up to passage 29 and authenticated by GTW banding karyotype method, only cells with normal 46, XY karyotype were used for experiments.

Techniques: Labeling, Staining, Expressing, Western Blot, Mutagenesis, Immunoprecipitation

Journal: Neuron

Article Title: Neuroligin-4 regulates excitatory synaptic transmission in human neurons

doi: 10.1016/j.neuron.2019.05.043

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Male human embryonic stem (ES) cells line H1 (WA01 WiCell Research Institute, Inc.) were cultured up to passage 29 and authenticated by GTW banding karyotype method, only cells with normal 46, XY karyotype were used for experiments.

Techniques: Recombinant, Reverse Transcription Polymerase Chain Reaction, Sequencing, Software